EVALUATION OF ANTIOXIDANT PROPERTIES OF A CO-POLYMERIZED HYDROGEL FROM NATURAL SOURCE FOR DIFFERENT BIOMEDICAL APPLICATIONS

Abstract

In order to introduce a material with improved antioxidant qualities, the current study focuses on the assessment of antioxidant properties of a co-polymerized hydrogel. By co-polymerizing the extracted seed mucilage of Mimosa pudica (MPH) with methacrylic acid (MA), a co-polymerized hydrogel (MPH-co-MA) with enhanced free radical scavenging capacity was created. The capacity of MPH-co-MA to lower oxidative stress was evaluated by measuring its antioxidant activity such as Frap, DPPH, TPC, and TFC, which makes it appropriate for use in tissue engineering, wound healing, and regulated medication delivery. FRAP values rose from 27.2–51.1 ????g AAE/mL spanning 100–500 µg/mL, and the extract demonstrated dose-dependent DPPH radical scavenging activity ranging from 10.77-37.53 µg. Strong antioxidant potential was confirmed by the gradual rise in phenolic and flavonoid levels, with TPC rising from 23.2-53.2 µg (GAE µg/mL) and TFC from 25.1-69.4, µg (QCE µg/mL). These results support the traditional uses of co-polymerized hydrogel by confirming that it has significant antioxidant effects.