ACUTE ORAL TOXICITY EVALUATION OF A CITRIC ACID-MEDIATED HYDROGEL ON RAT AS MODEL ANIMAL
DOI:
https://doi.org/10.66021/pakmcr650Keywords:
Polysaccharide based Hydrogel, Acute toxicological evaluation, non-irritating behavior, Clinical biochemical analysisAbstract
APIs plays a pivotal role in pharmaceutical development and must undergo comprehensive toxicological evaluation to establish their safety profile. In this study acute oral toxicity assessment of citric acid cross-linked hydrogel composed of chia seed mucilage were carried out in compliance with OECD guidelines 402 and 420, respectively. Rats were categorized into four groups, with one untreated control group, while the remaining groups received a single dose of composite hydrogel. Over 14 days of observation period, all animals survived without exhibiting any significant toxic effects. Hematological, biochemical and organs weight assessments confirmed the non-toxic nature of the hydrogel, supporting its potential use as a safe excipient in drug delivery system.
