COMPUTATIONAL ANALYSIS OF LIVER INFLAMMATION ASSOCIATED GENES IN HUMAN (HOMO SAPIENS) AND PHYLOGENY WITH CHIMPANZEE

Abstract

This study applies computational approaches to explore and characterize genes associated with liver inflammation in humans. Five key genes were analyzed: mitogen-activated protein kinase 1 (MAPK1), integrin subunit alpha 2 (ITGA2), cyclin-dependent kinase 2 (CDK2), interleukin 6 (IL6), and 2'-5'-oligoadenylate synthetase 2 (OAS2). The workflow included sequence retrieval from NCBI, chromosomal localization, gene structure analysis, motif identification, protein–protein interaction (PPI) mapping, and gene ontology annotation. Results indicated that the studied genes are dispersed across different chromosomes and play crucial roles in biological pathways linked to liver inflammation. PPI analysis highlighted ITGA2 as a hub gene with strong interaction connectivity. Gene ontology suggested that IL6 and OAS2 contribute significantly to antiviral defense mechanisms. Phylogenetic analysis showed a close relationship between OAS2-Hs and OAS2-Pt, whereas MAPK1-Hs displayed greater divergence. Expression profiling revealed that CDK2 is markedly upregulated in the telencephalon region. Overall, these findings provide new insights into the molecular mechanisms of liver inflammation and identify potential biomarkers for early diagnosis and therapeutic intervention, though experimental validation remains essential to support these computational predictions.