Screening And Characterization Of Ocular Infections Associated Bacterial Pathogens And Their Susceptibility Against Commonly Used Antibiotics

Abstract

Eye infections are wide spread and are associated with different bacterial pathogens. In this study, bacterial isolates were obtained from mild, moderate and severe eye infections samples. Samples were collected from LRBT (Layton Rehmat Benevolent Trust) and spread on agar plates. Morphological, biochemical and characterization of bacterial isolates was done. The bacteria identified from mild eye infections were Staphylococcus aureus and Haemophilus influenza I. Staphylococcus aureus and Pseudomonas aeruginosa were isolated from moderate ocula infections while from severe eye infections, Treponema primitia was isolated. The antibiotic sensitivity of these bacteria was also studied. All bacterial isolates showed resistance against Ampicillin. Bacterial isolates MS2B showed maximum sensitivity against Penicillin which was 11.5mm and bacterial isolates MS2A, MO1A and MO2 showed minimum sensitivity against Oxytetracycline which was 2.25mm. Optimum growth temperature observed was 37°C and pH 7. In current study, pathogenic bacteria isolated form eye infections showed resistance to commonly used antibiotics. It is because of misuse of antibiotics and self-medication resulted in resistance in pathogens against antibiotics. There is need to explore alternative ways to treat infections.