Evaluation Of Hepatoprotective Activity Of Thiourea Derivatives On Gentamycin Induced Hepatotoxicity
DOI:
https://doi.org/10.66021/pakmcr998Keywords:
TUD= Thio Urea Derivatives, H&E= Haematoxylin and Eosin resins, SGPT= Serum Glutamic Pyruvic Transaminase, ALT= Alanine aminotransferase, ALP= Alkaline Phosphatase, ROS= Reactive Oxygen Species, MDA= Malonaldehyde level, LPO= lipid peroxidation, CA= STO-II, CB= Psoduct-M, CC= Para-Thio-STP.Abstract
The vital organ liver performs many of functions in the body, including the production of bile, elimination of bilirubin, cholesterol, metabolism of drugs, fats, proteins, activation of enzymes, storage of glycogen, and synthesis of plasma proteins like albumin and clotting factors. Hepatotoxicity is a definitive aspect of liver repeatedly caused by exposure to xenobiotics such as drugs, alcohols, toxins and chemicals. Thiourea and thiourea derivatives are a well-known class of synthetic organosulfur compound also known as carbamides.
The present study was designed to elucidate the scientific background for the use of Thiourea derivatives as hepatoprotective agent. The compound was evaluated for the hepatoprotective activity in animal model by analyzing the blood biomarkers and examining the histological changes in the liver. For this purpose, the synthetic compound thiourea derivatives were prepared by Dr Wadood Ali Shah (Assistant Professor UOM). The dose of 50mg/kg and 250mg/kg from all the three compounds entitled as CA= STO-II, CB= Psoduct-M, CC= Para-Thio-STP were selected to evaluate the hepatoprotective effect. After dosing for 8 consecutive days, the blood samples were then analyzed for hepatic biomarkers such as Alanine aminotransferase (ALT) / Serum Glutamic Pyruvic Transaminase (SGPT) blood serum along with Alkaline Phosphatase ALP levels and the histology of liver. The blood serum levels of SGPT and the level of ALP were highly significantly lowered down by TUD in higher dose level of 250mg/kg, while the microscopic examination of liver histology revealed that TUD has improved clogged blood vessels, fatty changes, and inflammatory infiltrate markedly, induced by gentamicin.
Therefore, it was concluded from the findings of present study that thiourea derivatives has marked dose dependent hepatoprotective effect. As the literature review has declared that TUD constitutes different group of chemicals such as thiocarbonyl which possess antioxidant effect thus it might be concluded that the hepatoprotective effect of TUD may be due to thiocarbazone present in it.
