Impact Of Weight Training With Vitamin Supplementation On Liver Enzymes And Uric Acid Among Females With Non-Alcoholic Fatty Liver Disease

Abstract

Non-alcoholic fatty liver disease (NAFLD) is an increasingly prevalent metabolic disorder strongly associated with hyperuricemia, obesity, and insulin resistance, particularly among adult females. Elevated serum uric acid (SUA) contributes to oxidative stress and hepatic inflammation, accelerating NAFLD progression. Lifestyle-based interventions such as resistance exercise and micronutrient supplementation are emerging as effective non-pharmacological strategies. This study aimed to examine the combined effects of structured weight training and vitamin supplementation on SUA levels and liver function markers in females diagnosed with NAFLD.A randomized controlled experimental design was employed involving adult females (n = 20; 20–50 years) with ultrasound-confirmed NAFLD. Participants were randomly allocated into an experimental group (EG; weight training plus vitamin supplementation) or a control group (CG; routine physical activity). The intervention lasted eight weeks. Pre- and post-intervention assessments included body mass index (BMI), serum uric acid, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin. Data were analyzed using paired and independent samples t-tests. The experimental group demonstrated statistically significant reductions in serum uric acid (Δ −1.21 mg/dL, p < .01), ALT (Δ −14.3 U/L, p < .01), AST (Δ −11.6 U/L, p < .05), ALP (Δ −18.4 U/L, p < .01), and BMI (Δ −1.9 kg/m², p < .05) compared to the control group. No adverse events were reported. Eight weeks weight training and vitamin supplementation significantly improved uric acid regulation and hepatic enzyme profiles in females with NAFLD. These findings support the use of integrated lifestyle-based interventions as effective therapeutic strategies for NAFLD management.