Growth Optimization and Diagnosis of Helicobacter pylori in Tehsil Kulachi, Dera Ismail Khan, KPK

Abstract

1. pylori is a gram-negative bacterium which is mostly linked to the occurrence of peptic ulcers and gastric cancer. The present paper concentrated on Kulachi Tehsil of the Dera Ismail Khan District in the Khyber Pakhtunkhwa Province where the prevalence of H. pyloriis high, but the access to testing is limited. Based on these, we undertook a research to research on the new techniques used in growing H. pylorias well as to assess the Linear H. pylori Ab Cassette to be used in quick and effective diagnosis of the bacterium in various parts of the world. To make a comprehensive study of different parts of the Tehsil, we collected 200 samples of Kulachi and its vicinity villages namely: Hathala, Maddi, Rori, and Loni. The accuracy and effectiveness of the results were measured by both traditional methods and Linear H. pylori Ab Cassette. The researchers have reported an average H. pylori infection rate of 50 per cent (100/200 samples) and highest prevalence of H. pylori in Rori village (62.5 per cent) and in older people (75 per cent in 51+ age group) which has proven that the Linear H. pylori Ab Cassette was efficient in diagnosing H. pylori infection quickly. This article is important because it concentrates on the betterment of laboratory conditions in the context of H. pylori and also dwells on a powerful tool that could enable the easier identification of the same in the rural areas. This study is aimed at improving the process of diagnosis, reducing the time of tests, and consequently leading to better means of handling the disease related to H. pylori in the society. Hopefully, our results will initiate more efforts in the field of public health and make H. pylori more well-known so that more people in Tehsil Kulachi would have better health outcomes.

Keywords: Helicobacter pylori, Growth optimization, Bacterial diagnosis, Microbiological analysis, Clinical isolates